目的　分析赫坎按蚊种群内 4个近缘种核糖体基因内转录第二间隔区 (rDNAITS2 )特征。　方法　采用特异性ITS2 引物对中华按蚊和嗜人按蚊江苏实验株、辽宁省沈阳市和朝鲜开城现场捕获的嗜人按蚊、八代按蚊和雷氏按蚊rDNAITS2 进行PCR扩增、基因测序和限制性内切酶酶切位点图谱分析。　结果与结论　赫坎按蚊种群近缘种中华按蚊、嗜人按蚊、八代按蚊和雷氏按蚊rDNAITS2 分别为 472、45 2、45 6和 45 6bp ,各基因序列存在明显差异并存在不同的限制性内切酶酶切位点 Objective To analyze the characteristics of rDNAITS2 in the four closely related species of Hercian genus Hercán. Methods The specific ITS2 primers were used to amplify the anopheles anthropophagus, Anopheles stephensi and Anopheles stephensi rDNAITS2 collected from Jiangsu An. Sinensis and Anopheles anthropophagus, Shenyang, Liaoning Province and Kaesong, North Korea. The gene Sequencing and restriction endonuclease analysis. RESULTS AND CONCLUSION rDNAITS2 of Anopheles sinensis, Anopheles anthropophagus, Anopheles choriatae and An. Reesei were 472, 452, 456 and 456 bp, respectively, with significant differences in the gene sequences Different restriction endonuclease sites