论文部分内容阅读
目的:探讨子宫腺肌病中孕激素受体B(progesterone receptor B,PR-B)表达及其甲基化在疾病发生中的作用。方法:用免疫组化法测定PR-B的表达,甲基化特异性基因扩增(methylation-specific PCR,MSP)方法检测50例子宫腺肌病患者在位与异位内膜和18例输卵管不孕或良性卵巢囊肿患者子宫内膜中PR-B的甲基化状态,并分析甲基化与PR-B表达的相关性。结果:子宫腺肌病患者在位和异位内膜PR-B表达明显低于对照内膜(P<0.01);子宫腺肌病异位内膜中PR-B甲基化比率显著高于对照内膜(P<0.05);且子宫腺肌病在位和异位内膜中PR-B甲基化状态与PR-B表达相关(P<0.05)。结论:PR-B低表达及其甲基化可能与子宫腺肌病的发生有关。
Objective: To investigate the role of progesterone receptor B (PR-B) expression and its methylation in the pathogenesis of adenomyosis. Methods: The expression of PR-B was detected by immunohistochemistry. The methylation-specific PCR (MSP) was used to detect the expression of PR-B in ectopic endometrium and ectopic endometrium of 50 cases of adenomyosis and 18 cases of fallopian tube The methylation status of PR-B in endometrium of infertility or benign ovarian cysts was analyzed, and the correlation between methylation and PR-B expression was analyzed. Results: The expression of PR-B in eutopic and ectopic endometrium of patients with adenomyosis was significantly lower than that of control (P <0.01). The methylation of PR-B in ectopic endometrium of adenomyosis was significantly higher than that of control (P <0.05). The methylation status of PR-B in eutopic and ectopic endometrium of adenomyosis was correlated with PR-B expression (P <0.05). Conclusion: The low expression of PR-B and its methylation may be related to the occurrence of adenomyosis.