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在头发角化期仍在留许多药物痕迹,并在头发中存留着。头发中缺乏药物代谢,而头发以约1cm/月的相当一致的速度生长,可提供分析头发标本的用药史。将头发标本(全长)从头发根部分为2cm 一段。将每段头发称重,再在55℃用盐酸浸泡过夜,抽提头发中游离吗啡。中和后,用固相放射免疫法分析抽提物。在固定时间内~(125)Ⅰ标记吗啡与标本中的吗啡竞争吗啡特异抗体结合位点,然后在γ-计数仪中测定分离的抗体部分。测定标本同时作一测定吗啡的标
In the hair keratosis period still leaving many traces of medicine, and remain in the hair. Lack of drug metabolism in the hair while the hair grows at a fairly consistent rate of about 1 cm / month provides a history of drug use for analyzing hair samples. The hair samples (full length) from the root part of the hair is 2cm section. Weigh each piece of hair, and then soaked in hydrochloric acid at 55 ℃ overnight, extracted hair free morphine. After neutralization, the extracts were analyzed by solid-phase radioimmunoassay. At a fixed time, ~ (125) I labeled morphine competes for morphine-specific antibody binding sites with morphine in the specimen and the isolated antibody fraction is then measured in a gamma-counter. Determination of specimens at the same time for a determination of morphine standard