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本实验采用一次腹腔注射法中毒氚水。氚水注入量分别为3.22,6.44,11.06,21.76和31.23×10~2KBq/g.b.w。小鼠睾丸组织65天累积吸收剂量分别为0.070,0.140,0.242,0.476和0.684 Gy。中毒后65天处死动物。通过观察初级精母细胞D-MI中的多价体探讨氚水诱发精原细胞染色体易位的剂量效应关系。实验结果表明,易位率随氚水中毒剂量的增加而升高,其剂量效应关系可拟合为直线方程Y=0.581+0.0366X。精原细胞易位的产物主要为D-MI中的链状四价体,其占总畸变的77.8％;其次为链状六价体和锈状三价体+单价体,分别占总畸变的11.1％和9.7％;环状四价体最少,仅占1.4％。
In this experiment, an intraperitoneal injection of toxic tritiated water. Tritium water injection volume were 3.22,6.44,11.06,21.76 and 31.23 × 10 ~ 2KBq / g.b.w. The cumulative absorbed doses of mouse testis tissue at 65 days were 0.070, 0.140, 0.242, 0.47 and 0.684 Gy, respectively. The animals were sacrificed 65 days after poisoning. To investigate the dose-response relationship of tritiated water-induced chromosomal translocation in spermatogonia by observing polyplexes in D-MI of primary spermatocytes. The experimental results show that the translocation rate increases with the dose of tritiated water poisoning, and its dose-response relationship can be fitted as a straight line equation Y = 0.581 + 0.0366X. The products of spermatogonia translocation were mainly chain-like tetrads in D-MI, accounting for 77.8% of the total aberrations; followed by the chains of hexavalent and rusty trivalent + monovalent, accounting for the total aberrations 11.1% and 9.7% respectively; the least cyclic tetravalent species accounted for only 1.4%.