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目的观察β3肾上腺素能受体激动剂BRL37344对3T3-L1前脂肪细胞分化及相关基因mRNA转录的影响,并探讨其抗肥胖的作用机制。方法将3T3-L1前脂肪细胞分为4组:空白对照组、常规分化组、BRL37344处理组、常规分化+BRL37344组。采用油红O染色法初步判断各组脂肪细胞的分化情况,RT-PCR法检测各组脂肪细胞分化过程中过氧化物酶体增殖物激活受体γ2(Peroxisome proliferators activated receptorγ2,PPARγ2)、脂肪细胞型脂肪酸结合蛋白(Adipocyte fatty acidbinding protein,aP2)、脂蛋白脂肪酶(Lipoprotein lipase,LPL)基因mRNA的转录水平。结果经不同浓度的BRL37344处理8 d后,3T3-L1前脂肪细胞中PPARγ2、aP2、LPL基因mRNA的转录水平均下调,且呈剂量依赖性,其中10-7mol/L BRL37344作用明显;在不同的分化时间点,经10-7mol/L BRL37344处理后,细胞分化相关基因与相应对照组相比,均呈下调趋势(P<0.05)。结论β3肾上腺素能受体激动剂BRL37344可能通过下调脂肪细胞分化相关基因的转录,抑制3T3-L1前脂肪细胞的分化。
Objective To observe the effect of β37 adrenergic receptor agonist BRL37344 on the differentiation of 3T3-L1 preadipocytes and related gene mRNA transcription and to explore the mechanism of anti-obesity. Methods 3T3-L1 preadipocytes were divided into 4 groups: blank control group, normal differentiation group, BRL37344 treatment group and normal differentiation + BRL37344 group. Oil red O staining was used to determine the differentiation of adipocytes. The expression of peroxisome proliferators activated receptor γ2 (PPARγ2), adipocytes Transcriptional level of mRNA of aP2 and LPL in rats. Results The mRNA levels of PPARγ2, aP2 and LPL in 3T3-L1 preadipocytes were all down-regulated in a dose-dependent manner after treated with different concentrations of BRL37344 for 8 days, of which 10-7 mol / L BRL37344 had a significant effect. Differentiation time points, compared with the corresponding control group, the gene expression of cell differentiation decreased after treatment with 10-7mol / L BRL37344 (P <0.05). Conclusion β37 adrenergic receptor agonist BRL37344 may inhibit the differentiation of 3T3-L1 preadipocytes by down-regulating the transcription of adipocyte differentiation-related genes.