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目的探讨早期诊断肺炎支原体感染的方法。方法对350例14岁以下的疑似肺炎支原体上呼吸道感染的患者进行血清金标渗滤法检测肺炎支原体抗体,同时用咽拭子标本进行肺炎支原体(MP)核酸扩增(PCR)荧光定量检测法检测标本中的肺炎支原体病原体(DNA)。结果金标渗滤法检测350例上呼吸道感染MP-IgM 76例阳性,阳性率为21.7%。MP-IgG有56例阳性,阳性率为16%。PCR法检测结果MP-DNA有150例阳性,阳性率是42.9%。结论荧光定量PCR法检测在早期诊断肺炎支原体感染优于金标渗滤法,是早期诊断肺炎支原体感染的金标准。
Objective To explore the early diagnosis of Mycoplasma pneumoniae infection. Methods 350 patients with suspected pneumonia mycoplasma pneumonia under 14 years of age were tested for Mycoplasma pneumoniae antibody by serum gold standard. Meanwhile, throat swab samples were used for quantitative PCR detection of Mycoplasma pneumoniae (MP) Mycoplasma pneumoniae pathogen (DNA) in specimens. Results The gold standard of percolation method detected 350 cases of upper respiratory tract infection MP-IgM 76 cases positive, the positive rate was 21.7%. 56 cases of MP-IgG positive, the positive rate of 16%. PCR results of MP-DNA in 150 cases were positive, the positive rate was 42.9%. Conclusion The detection of mycoplasma pneumoniae by fluorescence quantitative PCR is superior to gold standard percolation in the early diagnosis, which is the gold standard for the early diagnosis of Mycoplasma pneumoniae infection.