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目的 探索天然抗角蛋白自身抗体 (AK auto Ab)对培养角质形成细胞及鳞癌细胞凋亡相关蛋白的表达影响 .方法 经体外培养人角质形成细胞及鳞癌细胞 ,以不同滴度的天然抗角蛋白自身抗体作用 ,并用 MTT法及免疫组化检测 .结果 AK auto Ab对二者的增殖有着明显的抑制作用 ,且呈一定的剂量 -效应关系 .可促进二者凋亡相关蛋白 c- Myc的表达 ;对 Bcl- 2在 SCC中的表达表现为抑制作用 ,而对其在KC中的表达作用却不明显 .结论 天然抗角蛋白自身抗体对培养角质形成细胞及鳞癌细胞的增殖起着抑制作用 ,并可能通过调节凋亡相关蛋白的表达来实现
Objective To explore the effect of natural keratin autoantibody (AK auto Ab) on the expression of apoptosis-related proteins in cultured keratinocytes and squamous cell carcinoma.Methods Human keratinocytes and squamous cell carcinoma cells were cultured in vitro with different titres of natural anti- Keratin autoantibodies and detected by MTT assay and immunohistochemistry.Results AK auto Ab significantly inhibited the proliferation of both cells and showed a dose-response relationship.It could promote the expression of c-Myc Expression of Bcl-2 in SCC but not in KC.Conclusion The effect of natural keratin autoantibodies on the proliferation of keratinocytes and squamous cell carcinoma Inhibition, and may be through the regulation of apoptosis-related protein expression to achieve