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为进一步探讨动脉粥样硬化形成及血管成形术后再狭窄的发生机制，应用光镜、显微-微机图像分析、发色底物显色法活性测定、原位分子杂交等技术，研究兔血管损伤后不同时间组织型纤溶酶原激活物（t-PA）在平滑肌细胞（SMC）的表达。研究发现：血管损伤后4天t-PA活性明显增加[（7．50±0．05）×10-2IU／mg蛋白]，此时SMC开始从中膜向内膜迁移。损伤后7天，t-PA活性降至近于正常水平[（2．50±0．37）×10-2IU／mg蛋白]。正常血管壁t-PAmRNA杂交阴性，血管损伤后4天，内弹力板下及内膜SMC有大量t-PAmRNA表达，7天时仅表达少量。说明t-PA可能在血管损伤后的SMC增生和迁移过程中起重要作用。
In order to further explore the mechanism of atherosclerosis and restenosis after angioplasty, using light microscopy, micro-computer image analysis, color chromogenic substrate activity assay, in situ hybridization and other techniques, Expression of tissue-type plasminogen activator (t-PA) in smooth muscle cells (SMCs) at different times after injury. The study found that: 4 days after vascular injury t-PA activity was significantly increased [(7.50 ± 0.05) × 10-2IU / mg protein], when SMC began to migrate from the medial to the intima. At 7 days after injury, t-PA activity decreased to near-normal levels (2.50 ± 0.37 × 10 -2 IU / mg protein). Normal blood vessel wall t-PA mRNA negative hybridization, 4 days after vascular injury, endometrial and endometrial SMC have a lot of t-PA mRNA expression, only a small amount of expression on the 7th day. This suggests that t-PA may play an important role in the proliferation and migration of SMCs after vascular injury.