论文部分内容阅读
目的 建立定量测定红细胞CD44s和CD5 8分子的实验方法 ,研究其数量变化与肿瘤转移的关系。方法 将戊二醛固定的 1.2× 10 6红细胞定量“液相包被”于V型板中 ,依次加入抗CD44s和CD5 8分子单克隆抗体、碱性磷酸酶标记的第二抗体及可溶性底物 ,移显色液于比色孔 ,在40 5nm比色 ,计算其A40 5吸光度值。结果 本方法具有良好的敏感性和重复性。非转移性肿瘤患者红细胞CD44s和CD5 8分子数量表达低于正常人群 ,但无统计学差异。而转移性卵巢癌和肝癌患者的红细胞CD44s和CD5 8分子数量明显低于无转移肿瘤患者和正常人群 (P <0 .0 0 1)。CD44s数量变化与肿瘤患者血清透明质酸 (HA)含量变化密切相关。红细胞CD5 8分子表达变化对红细胞调节淋巴细胞免疫粘附能力有明显影响。结论 红细胞CD44s分子数量表达变化与肿瘤转移密切相关 ;红细胞CD5 8分子参与机体整体免疫调节
Objective To establish an experimental method for quantitative determination of red blood cell CD44s and CD5 8 molecules, and to study the relationship between their quantitative changes and tumor metastasis. METHODS: Quantitative “liquid coating” of glutaraldehyde-fixed 1.2 × 10 6 erythrocytes in V-plates was performed by sequential addition of anti-CD44s and CD58 monoclonal antibodies, alkaline phosphatase-labeled secondary antibodies, and soluble substrates. The color liquid was transferred to the colorimetric wells and compared at 40 5 nm to calculate the A40 5 absorbance value. Results The method has good sensitivity and repeatability. The number of red blood cell CD44s and CD5 8 molecules in patients with non-metastatic tumors was lower than in the normal population, but there was no statistical difference. The number of red blood cell CD44s and CD58 molecules in patients with metastatic ovarian and hepatic cancer was significantly lower than that in patients without metastatic tumors and normal controls (P < 0.01). The change of the number of CD44s is closely related to the change of serum hyaluronic acid (HA) content in cancer patients. The change of red blood cell CD5 8 expression has a significant effect on the ability of red blood cells to modulate lymphocyte immune adherence. Conclusion The expression of CD44s in erythrocytes is closely related to tumor metastasis. Red blood cell CD5 8 is involved in the overall immune regulation of the body.