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目的对于孕妇尿液进行HCMV-DNA筛查,减少和有效地避免胎儿及新生儿的HCMV感染。HCMV-DNA筛查同时进行HCMV抗体检测,明确诊断HCMV感染的灵敏、准确方法。方法应用荧光定量PCR(FQ-PCR)方法进行尿液HCMV-DNA测定。血液HCMV IgM、IgG抗体检测应用酶联免疫(ELISA)方法。结果筛查6568例孕妇尿HCMV-DNA,阳性273例,阳性率为4.2%。孕妇在12~20周者,阳性率为10.3%;孕妇在21~30周者,阳性率为33.3%;孕妇在31~39周者,阳性率为56.4%。在273例尿液HCMV-DNA阳性者中,56例同时进行血液HCMV IgM、IgG抗体检测,IgM抗体阳性者2例,IgG抗体阳性者22例。结论在孕妇HCMV感染的筛查与诊断中FQ-PCR是灵敏准确的方法。孕妇中HCMV-DNA筛查是保证母婴健康,提高人口素质的保障。
Objective To screen HCMV-DNA in pregnant women’s urine to reduce and effectively prevent HCMV infection in fetuses and newborns. HCMV-DNA screening at the same time detection of HCMV antibodies, a clear diagnosis of HCMV infection sensitive, accurate method. Methods Fluorescence quantitative PCR (FQ-PCR) was used to detect HCMV-DNA in urine. Blood HCMV IgM, IgG antibody detection using enzyme-linked immunosensing (ELISA) method. Results The urine HCMV-DNA of 6568 pregnant women was screened, with a positive rate of 4.2%. The positive rate was 10.3% in pregnant women between 12 and 20 weeks, and the positive rate was 33.3% in pregnant women between 21 and 30 weeks. The positive rate was 56.4% in pregnant women between 31 and 39 weeks. Among 273 HCMV-DNA positive cases, 56 cases were tested for blood HCMV IgM and IgG antibody, 2 cases were positive for IgM antibody and 22 cases were IgG antibody positive. Conclusion FQ-PCR is a sensitive and accurate method for the screening and diagnosis of HCMV infection in pregnant women. HCMV-DNA screening in pregnant women is to ensure the health of mothers and infants, improve the quality of the population protection.