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从香蕉cDNA文库中克隆到了一个香蕉(Musa acuminata AAA subgroup)乙二醛酶(glyoxalase,GLO)基因(MaGLO14)。构建了带有MaGLO14的酵母表达载体PYES2-MaGLO14,转化酿酒酵母(Saccharomyces cerevisiae)尿嘧啶营养缺陷型菌株INVSC1,挑取转化子进行PCR和酶切鉴定,证实获得了转基因菌株。通过比较转基因菌株和非转基因菌株在NaCl、高温、低温、干旱、UV胁迫下的生长状况,证明转基因菌株在以上非生物胁迫条件下的存活菌落数均高于非转基因菌株。利用酿酒酵母初步证明MaGLO14具有增强酵母菌对非生物胁迫抵抗力的功能。
A banana (Musa acuminata AAA subgroup) glyoxalase (GLO) gene (MaGLO14) was cloned from banana cDNA library. The yeast expression vector PYES2-MaGLO14 with MaGLO14 was constructed and the uracil auxotrophic strain INVSC1 was transformed into Saccharomyces cerevisiae. The transformants were selected for PCR and restriction enzyme digestion, and the transgenic strains were confirmed. By comparing the growth status of transgenic and non-transgenic strains under NaCl, high temperature, low temperature, drought and UV stress, the number of viable colonies of the transgenic strains under the above abiotic stress conditions was higher than that of non-transgenic strains. Preliminary studies using Saccharomyces cerevisiae showed that MaGLO14 has the function of enhancing the resistance of yeast to abiotic stress.