1998年11月在长江口及周围海域进行了一个航次的环境调查，并在室内以中肋骨条藻为实验藻种，应用营养加富生物测定法对本海域浮游植物的营养需求状况进行了研究．结果表明，可大致根据离河口的远近将此海域分为3个部分：近河口光限制区、过渡带光和磷酸盐限制区、远河口氮盐限制区．P与N分别为潜在营养限制因子的界线大致沿盐度为31的等盐线．并指出，以溶解无机氮磷比的判断标准，在悬浮物浓度较高的区域会高估磷的限制作用． In November 1998, a voyage environment survey was conducted in the Changjiang River estuary and its surrounding sea areas. In the laboratory, Skeletonema costatum was used as experimental algal species. The nutrient requirements of phytoplankton in this area were studied by nutrient-rich bioassay. The results show that the sea area can be roughly divided into three parts according to the distance of estuaries: light near the mouth of the river, light of the transitional zone and phosphate, and salt restriction of the far estuary. P and N are the iso-salt lines along the salinity of 31, respectively, for potential nutrition restriction factors. And pointed out that to determine the ratio of dissolved inorganic nitrogen and phosphorus in the higher concentrations of suspended matter will overestimate the phosphorus limit.