目的评价不同染色法的精子形态学分析结果。方法用改良巴氏、Shorr、Diff-Quik及瑞-吉氏染色法对50例精液涂片染色,分别计算精子形态正常率、头部畸形率、中段畸形率、尾部畸形率及顶体完整率。结果 4种染色方法检测形态正常精子率和头部畸形精子率差异均无统计学意义(P>0.05)。瑞-吉氏染色法检测中段及尾部畸形精子率明显低于其它染色法(P<0.05)。检测顶体完整精子率时,瑞-吉氏及Shorr染色法相对更易区分顶体与核。结论 4种精子形态染色方法均可作为人类精子形态学检测实用方法。其中,Shorr染色法快速、简单,能较好区分精子各部分形态,且适用于顶体完整率判读。 Objective To evaluate the results of sperm morphology analysis by different staining methods. Methods 50 cases of semen smears were stained with modified Papanicolaou, Shorr, Diff-Quik and Reiki-Giemsa staining. The sperm morphology normal rate, head deformity rate, middle malformation rate, caudal deformity rate and acrosome integrity rate . Results There was no significant difference between the four kinds of staining methods in detecting morphologically normal sperm ratio and head abnormal sperm ratio (P> 0.05). The Rui-Kyrgyz staining showed that the rate of abnormal sperm in the middle and tail were significantly lower than those in other methods (P <0.05). When detecting the intact spermatozoa rate, Ray-Kyrgyzstan and Shorr staining method is relatively easier to distinguish between the acrosome and the nucleus. Conclusion All four sperm morphology staining methods can be used as a practical method for human sperm morphology detection. Among them, Shorr staining fast, simple, can better distinguish the various parts of sperm morphology, and for acromiocutaneous rate of interpretation.