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目的:揭示肝癌诱发过程中肝组织基因表达谱的演变,为中医药防治原发性肝癌提供参考。方法:采用DEN诱发大鼠肝癌,分别于诱癌的第4周、8周、16周、20周(肝癌形成)切取肝(合肝癌)组织,常规提取RNA,Affymetrix Rat 230A GeneChip及技术检测大鼠肝组织基因表达的差异和演变。结果:在芯片的15710个基因中,正常组有9225个基因表达,诱癌4周表达增至9396个,8周增至9872个,16周增至10496个,20周有10420个。在肝癌诱发过程中,存在大量基因表达的消长以及高表达基因数的增加,其中部分已知基因的结果。结论:DEN诱发大鼠肝癌过程中基因组变化是十分复杂的,而且文献追踪表明,国内外对在这些基因的功能及其与肝癌发生、发展的关系大多不明确。因此,如何逐一找到那些起着关键作用的基因,进一步阐释其在肝癌的发生、发展和转归中的作用,及其与中医证候演变和相应治法的关系,是今后中医基础理论实验研究的重要方向。
Objective: To reveal the evolution of liver cancer gene expression profile induced by hepatocellular carcinoma (HCC) and to provide reference for prevention and treatment of primary liver cancer by Chinese medicine. Methods: The hepatocellular carcinoma of rat was induced by DEN. Liver (HCC) tissues were excised at the 4th, 8th, 16th and 20th weeks of carcinogenesis (hepatocellular carcinoma), routinely extracted RNA, Affymetrix Rat 230A GeneChip, Difference and evolution of gene expression in murine liver tissue. RESULTS: Of the 15 710 genes in the chip, 9,225 genes were expressed in the normal group and 9,396 in the 4 weeks of cancer induction, 9872 in 8 weeks, 10,496 in 16 weeks and 10,420 in 20 weeks. During the induction of liver cancer, there is a large number of gene expression and decline in the number of high expression of genes increased, some of which are known gene results. CONCLUSION: Genomic changes induced by DEN in rat hepatocellular carcinoma are very complex. Moreover, the literature review shows that the relationship between the function of these genes and the occurrence and development of hepatocellular carcinoma is not clear at home and abroad. Therefore, one by one to find those genes that play a key role, to further explain its role in the occurrence, development and prognosis of liver cancer, and its relationship with the evolution of TCM syndrome and the corresponding governing law is the future of basic theory of traditional Chinese medicine research The important direction.