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目的了解登封市1株引起幼儿腹泻的小肠结肠炎耶尔森菌的血清型、生物型、毒力基因携带情况。方法通过细菌培养、血清凝集法及PCR检测技术对分离菌株进行血清分型、毒力基因检测。结果从幼儿采集的粪便标本中检出小肠结肠炎耶尔森菌,镜下见革兰染色阴性短杆菌。该菌氧化酶阴性,触酶阳性,无硫化氢产生,尿素酶试验阳性,ONPG(邻硝基酚-半乳糖苷酶)试验阳性,血清分型为O∶3,生物型为3型。该菌株O∶3血清型阳性,生物型3型,毒力基因ail+、yst A+、yst B-、yad A+、vir F+。结论引起幼儿腹泻的病原菌为致病性小肠结肠炎耶尔森菌。
Objective To understand the serotypes, biotypes and virulence genes of Yersinia enterocolitica, a strain of enterocolitis causing childhood diarrhea in Dengfeng city. Methods The bacterial strains, serum agglutination method and PCR assay were used to detect the serotypes and virulence genes of the isolates. Results Yersinia enterocolitica was detected in stool samples collected from young children. Gram-negative bacteria were found in the microscope. The bacterium had negative oxidase, positive catalase, no hydrogen sulfide, positive urease test and positive ONPG (Ortho-Nitrophenol-Galactosidase) test. The serotype was O: 3 and the biotype was 3. The strain O: 3 seropositive, biotype 3, virulence genes ail +, yst A +, yst B-, yad A +, vir F +. Conclusion The pathogen causing diarrhea in young children is Yersinia enterocolitica.