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目的 :研究六味地黄丸对体外培养的黑素细胞的抑制作用。方法 :采用MTT法测定细胞增殖情况 ;NaOH裂解法测定黑素合成 ;Takahashi法测定酪氨酶含量。结果 :六味地黄丸对黑素细胞的增殖有抑制作用 ,能使细胞数明显减少 (P<0 .0 5 ) ,并能使黑素合成显著下降 (P <0 .0 1) ,使酪氨酸酶活性逐渐减弱 (P <0 .0 1)。结论 :六味地黄丸能抑制黑素细胞增殖、黑素合成、酪氨酸酶活性 ,这可能是临床应有六味地黄丸治疗皮肤色素性疾病的机制。
Objective: To study the inhibitory effect of Liuweidihuang Pills on melanocytes cultured in vitro. METHODS: MTT assay was used to determine cell proliferation, NaOH lysis was used to determine melanin synthesis, and Takahashi was used to determine tyrosinase content. RESULTS: Liuwei Dihuang Pills inhibited the proliferation of melanocytes, significantly reduced the number of cells (P<0.05), and significantly decreased the synthesis of melanin (P < 0.01). Acidase activity gradually decreased (P < 0.01). Conclusion : Liuweidihuangwan can inhibit melanocyte proliferation, melanin synthesis and tyrosinase activity, which may be the mechanism of clinical treatment of pigmentary diseases with Liuwei Dihuang Wan.