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286例广西地区京族人群无关个体血样,采用Chelex-100的方法提取DNA,荧光标记复合扩增系统Profiler Plus Kit(ABI公司)扩增D8S1179、1321S11、D18S51、D3S1358、 VWA、 FGA、D5S818、D13S317、D7S820等9个STR基因座,ABI 377-96全自动测序仪对扩增产物进行检测,GenoTyper软件进行基因分型。统计处理所获得的数据资料,为个人识别、亲权鉴定、DNA数据库技术等法医学应用及群体遗传学、民族学提供基础资料,现报告如下。
286 cases of unrelated individual blood samples were collected from the population of Jing nationality in Guangxi province. The DNA was extracted with Chelex-100, and the DNA of D8S1179, 1321S11, D18S51, D3S1358, VWA, FGA, D5S818 and D13S317 were amplified by the fluorescent labeling multiplex amplification system Profiler Plus Kit (ABI) D7S820 and other 9 STR loci, ABI 377-96 automated sequencer to detect amplification products, GenoTyper software for genotyping. Statistical data obtained from processing, for personal identification, paternity testing, DNA database technology and other forensic applications and population genetics, ethnics to provide basic information, are as follows.