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以一年生油茶容器苗为试验材料,采用称重法控制基质含水量,设置CK,T1,T2,T3,T4和T5 6个水分处理组,研究水分胁迫下容器苗的生理生化特征差异。结果表明:(1)CK组的叶片含水量和自由水/束缚水值都为最大,分别为68.5%和26.4,而其他4组随着基质含水量减少其叶片含水量和自由水/束缚水值逐渐减小。(2)相对电导率、过氧化物酶活性和丙二醛含量都随着基质含水量的减少而呈先减后增的趋势,其最低值拐点出现在T1组,分别为37%、11.1 U·min-1·g-1和0.3μg·g-1。(3)游离脯氨酸和可溶性糖的含量随着基质含水量的减少而增加,由CK组的231.4μm·g-1和40.4 mg·g-1增加到T4组的681.4μg·g-1和48.9 mg·g-1。综上所述,81%～90%的基质含水量最适合油茶容器苗木生长;当基质含水量超过90%时,叶片气孔开度减小,不利于苗木光合作用和呼吸作用等生理过程;当基质含水量下降至81%以下时,苗木开始受到干旱胁迫。在今后的容器育苗生产中,应保持基质合适的水分含量,以维持其正常生长。
The annual seedlings of Camellia oleifera were used as materials to control the water content of matrix by using the weighing method. Six water treatment groups of CK, T1, T2, T3, T4 and T5 were set up to study the difference of physiological and biochemical characteristics of container seedlings under water stress. The results showed that: (1) The leaf water content and free water / irreducible water of CK group were the highest, which were 68.5% and 26.4, respectively. In the other four groups, the leaf water content and free water / irreducible water Value decreases gradually. (2) The relative conductivity, peroxidase activity and malondialdehyde content decreased first and then increased with the decreasing of substrate water content, and the lowest inflection point appeared in T1 group, which was 37% and 11.1 U · Min -1 · g -1 and 0.3 μg · g -1. (3) The content of free proline and soluble sugar increased with the decrease of substrate water content, and increased from 231.4μm · g-1 and 40.4 mg · g-1 in CK group to 681.4μg · g-1 in T4 group And 48.9 mg · g -1. In summary, 81% ~ 90% of the substrate moisture content is most suitable for seedling growth of Camellia oleifera. When the substrate moisture content exceeds 90%, the stomatal opening of leaves is reduced, which is not conducive to physiological processes such as photosynthesis and respiration of seedlings. When the substrate water content drops below 81%, seedlings begin to be drought-stressed. In the future container seedling production, should maintain the appropriate moisture content of the matrix in order to maintain its normal growth.