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采用免疫级化S－P方法检测95例喉癌、10例声带息肉和6例喉正常声带组织的p53表达，结果显示，p53蛋白主要定位于喉癌细胞内，呈棕黄色阳性颗粒，少部分位于细胞浆内，95例中阳性显色42例，阳性率44．2％。10例喉声带息肉和6例喉正常声带组织均为阴性。喉癌中p53基因表达阳性与声带息肉、正常声带组织相比差异显著（P＜0．01）。喉癌组中，不同年龄组发病有显著性差异（P＜0．05）。喉癌不同临床分期的p53表达阳性有显著性差异（P＜0．05）。p53表达阳性与喉癌病理分化程度、临床分型、颈部淋巴结转移及局部复发无显著性差异（P＞0．05）。p53表达阳性组和阴性组术后生存曲线有明显不同（P＜0．05），p53表达阳性者术后生存期短、病死率增高。表明S－P免疫组化是检测喉癌p53基因表达的一种简便、灵敏、特异的方法。
Immunohistochemical S-P method was used to detect the expression of p53 in 95 cases of laryngeal carcinoma, 10 cases of vocal cord polyps, and 6 cases of normal vocal cord tissue. The results showed that p53 protein mainly localized in laryngeal carcinoma cells, showing brown-yellow positive particles. Located in the cytoplasm, 95 cases of positive coloration in 42 cases, the positive rate of 44.2%. 10 cases of laryngeal vocal cord polyps and 6 cases of normal vocal cord tissue were negative. The positive expression of p53 gene in laryngeal carcinoma was significantly different from that in vocal cord polyps and normal vocal cord tissues (P<0.01). In the laryngeal cancer group, there was a significant difference in incidence among different age groups (P<0.05). The positive expression of p53 in different clinical stages of laryngeal carcinoma was significantly different (P<0.05). There was no significant difference in the positive expression of p53 and pathological differentiation, clinical classification, lymph node metastasis and local recurrence of laryngeal carcinoma (P>0.05). The survival curve of the positive and negative p53 expression groups was significantly different (P<0.05). The patients with positive p53 expression had a short survival time and increased mortality. It shows that S-P immunohistochemistry is a simple, sensitive and specific method to detect p53 gene expression in laryngeal carcinoma.