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目的:探讨溶血标本对酶联免疫法检测HBsAg的影响。方法:选取2013年12月-2015年3月来我院进行治疗的患者126例,其中阴性标本51个,乙型肝炎患者标本75个,进行常规检测后认为造成溶血情况,使用酶联免疫法进行检测,观察HBsAg的具体情况。结果:经过检验后发现,溶血情况的发生对测定结果有一定的干扰,从乙肝患者的血液标本吸光度情况方面来看,受到的干扰较大,但是并不影响对疾病结果的判定,P>0.05,无显著性差异和统计学意义。结论:溶血标本对酶联免疫法检测HBsAg的影响不大,在血红蛋白小于10g/L的情况下并不会对异性肝炎病毒表面的抗原测定的结果产生影响,但是患者的标本产生溶血情况依然不能忽视。
Objective: To investigate the influence of hemolysis on the detection of HBsAg by enzyme-linked immunosorbent assay. Methods: Totally 126 patients were treated in our hospital from December 2013 to March 2015, including 51 negative samples and 75 hepatitis B virus samples. After routine tests, hemolysis was considered. Enzyme-linked immunosorbent assay For testing, observe the specific situation of HBsAg. Results: After the test, it was found that the occurrence of hemolysis had a certain interference with the determination results. From the aspects of the blood sample absorbance of hepatitis B patients, it was more disturbed, but it did not affect the determination of the disease outcome, P> 0.05 , No significant difference and statistical significance. Conclusion: Hemolysis samples have little effect on the detection of HBsAg by enzyme-linked immunosorbent assay (ELISA). Hemoglobin less than 10g / L does not affect the results of antigens on the surface of Hepatitis B virus, but hemolysis in patients still can not Neglect.