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目的:探讨谷胱苷肽转硫酶M1、T1、P1(GSTM1、GSTT1和GSTP1)基因多态性和吸烟饮酒习惯与直肠癌易感性的关系。方法:以直肠癌患者210例,人群对照439例为研究对象,调查研究对象的生活习惯,以多重PCR技术检测GSTM1和GSTT1基因缺失,PCR-RFLP技术检测GSTP1基因单核苷酸多态(第105密码子A→G)。结果:GSTM1和GSTT1基因缺失频率在病例组和对照组差异无显著性;GSTP1A/A、A/G和G/G基因型频率分布在病例组和对照组差异无显著性;与GSTP1A/A基因型携带者相比,G/G基因型者发生直肠癌的危险性无显著升高,调整OR值为1.11(95%CI:0.77～1.60)。结论:GSTM1、GSTT1和GSTP1基因多态性与直肠癌易感性无关。
Objective: To investigate the relationship between the polymorphisms of glutathione S-transferase M1, T1, P1 (GSTM1, GSTT1 and GSTP1) and smoking and drinking habits and the susceptibility to rectal cancer. Methods: A total of 210 patients with colorectal cancer and 439 controls were enrolled in this study. Their living habits were investigated. Multiplex PCR was used to detect the deletion of GSTM1 and GSTT1 gene. PCR-RFLP was used to detect single nucleotide polymorphisms 105 codon A → G). Results: The frequencies of GSTM1 and GSTT1 gene deletion were not significantly different between the case group and the control group. The frequencies of GSTP1A / A, A / G and G / G genotypes were not significantly different between the case group and the control group. There was no significant increase in the risk of rectal cancer in G / G genotypes with a adjusted odds ratio of 1.11 (95% CI: 0.77 to 1.60) compared with patients with type 2 carriers. Conclusion: The polymorphisms of GSTM1, GSTT1 and GSTP1 are not associated with susceptibility to rectal cancer.