The depression behavior and mechanism of guar gum on talc-type scheelite flotation were systematically investigated by flotation experiments, adsorption tests, zeta-potential measurements, and infrared spectroscopic analyses. The flotation results for monominerals, mixed minerals, and actual mineral samples indicated that guar gum exhibited much higher selective depression for talc than for scheelite. Bench-scale closed-circuit tests showed that a tungsten concentrate with a WO_3 grade of 51.43% and a WO_3 recovery of 76.18% was obtained. Adsorption tests, zeta-potential measurements, and infrared spectral analyses confirmed that guar gum absorbed more strongly onto the talc surface than onto the scheelite surface because of chemisorption between guar gum and talc. This chemisorption is responsible for the guar gum’s highly selective depression for talc and small depression for scheelite. The flotation results provide technical support for talc-type scheelite flotation. The depression behavior and mechanism of guar gum on talc-type scheelite flotation were systematically investigated by flotation experiments, adsorption tests, zeta-potential measurements, and infrared spectroscopic analyzes. The flotation results for monominerals, mixed minerals, and actual mineral samples indicated that guar Bench-scale closed-circuit tests showed that a tungsten concentrate with a WO_3 grade of 51.43% and a WO_3 recovery of 76.18% was obtained. Adsorption tests, zeta-potential measurements, and infrared spectral analyzes studies that guar gum absorbed more strongly onto the talc surface than onto the scheelite surface because of chemisorption between guar gum and talc. The chemisorption is responsible for the guar gum’s highly selective depression for talc and small depression for scheelite. The flotation results provide technical support for talc-type scheelite flotation.