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目的:研究GnRHⅡ对子宫内膜异位症(内异症)患者离体培养的异位及在位子宫内膜间质细胞凋亡的影响。方法:体外原代培养人内异症异位及在位子宫内膜间质细胞,用不同浓度的GnRHⅡ(0、10-10、10-8、10-6mol/L)干预,采用Hoechst染色和流式细胞术检测离体培养子宫内膜间质细胞的凋亡发生情况。结果:GnRHⅡ可诱导离体培养的在位或异位子宫内膜间质细胞的凋亡,随着浓度增加,差异有统计学意义(P<0.05),呈剂量依赖性;且对异位内膜间质细胞的促凋亡作用明显强于在位者(P<0.05)。结论:外源性GnRHⅡ可明显促进人内异症异位及在位子宫内膜间质细胞的凋亡,为开发内异症促凋亡治疗方面的新药提供了实验依据。
Objective: To study the effect of GnRHⅡ on the ectopic and endometrial stromal cell apoptosis in vitro in patients with endometriosis (endometriosis). Methods: Ectopic endometriosis and eutopic endometrial stromal cells were cultured in vitro. The cells were treated with different concentrations of GnRHⅡ (0, 10-10, 10-8, 10-6 mol / L) and Hoechst staining Flow cytometry was used to detect the apoptosis of cultured endometrial stromal cells in vitro. Results: GnRH Ⅱ induced apoptosis of eutopic and ectopic endometrial stromal cells in vitro, with a statistically significant difference (P <0.05), with a dose-dependent manner. The pro-apoptotic effect of mesenchymal cells was significantly stronger than that of those who were present (P <0.05). Conclusion: Exogenous GnRH Ⅱ can significantly promote the apoptosis of ectopic endometriosis and endometrial stromal cells in endometriosis and provide experimental basis for the development of new drugs in the treatment of endometriosis-induced apoptosis.