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作者从一窝白色ＩＣＲ远交系小鼠仔鼠群中发现突变裸鼠，采用回交一互交（ｂａｃｋ－ｉｎｔｅｒｃｒｏｓｓ）导入法，将“ｎｕ”基因保留下来，随后用隔离器饲养的ＩＣＲ小鼠为代乳鼠，通过剖腹取胎净化该种群，微生物学检查其主要项目达到ＳＰＦ级标准。术后动物饲养于洁净层流架中，￣（６０）Ｃｏ灭菌饲料，酸化水、鼠盒、垫料均高压灭菌，以纯合雄鼠（ｎｕ／ｎｕ）与杂合雌鼠（ｎｕ／＋）１：１非近亲繁殖，扩大了种群，定名ＩＣＲ／ＰＭＵ－ｎｕ裸鼠。与此同时，对突变裸鼠进行了免疫生物学检查，剖检１０余只不同鼠龄裸鼠，未见胸腺。观察７只突变棵鼠胸腺、脾及淋巴结的淋巴细胞转化实验，计算刺激指数，胸腺０．７１±０．５０、脾１．６０±０．６４、淋巴结０．８５±０．１３，三项均为ＳＩ＜２，证明裸鼠缺少Ｔ细胞，对刀豆素（ＣｏｎＡ）不起反应，而Ｂ淋巴细胞膜表面兔疫球蛋白（Ｓｍｌｇ￣＋）检查，与对照组无显著差异，显示Ｂ细胞正常。ＩＣＲ／ＰＭＵ－ｎｕ裸鼠用于动物实验，取得如下结果：裸鼠皮下肿瘤异种移植的成瘤结果与成瘤率分别为：人膀胱癌８／１０（８０％）、人胃癌２／２（１００％）、人结肠粘液腺癌２／２（１００％）、人肺巨细胞癌２／２（１００％）、人前列腺癌２?
The authors found mutant nude mice from a litter of white ICR outbred mice and retained the “nu” gene by back-intercross, followed by a small ICR The mice were on behalf of the suckling mice, which were purified by cesarean section and the main items of the microbiology examination reached the SPF standard. The postoperative animals were housed in a clean laminar flow frame and were sterilized with ~ (60) Co sterilized feed, acidified water, rat and litter, and homogenized with nu / nu and nu / +) 1: 1 Non-inbreeding, expanded population, naming ICR / PMU-nu nude mice. At the same time, the mutation of nude mice immunobiology, autopsy more than 10 different age mice, no thymic. The lymphocyte transformation experiments of thymus, spleen and lymph nodes in 7 mutant mice were observed. The stimulation index, thymus 0.71 ± 0.50, spleen 1.60 ± 0.64, lymph node 0.85 ± 0.13 were calculated, Both SI <2, proved lack of T cells in nude mice, ConA can not react, and B lymphocyte membrane surface immunoglobulin (Smlg ~ +) examination, no significant difference with the control group, showing that B cells normal. ICR / PMU-nu nude mice were used in animal experiments and the following results were obtained: The tumorigenic rates and tumorigenic rates of subcutaneous tumor xenografts in nude mice were 8/10 (80%) for human bladder cancer and 2/2 100%), human colon mucinous adenocarcinoma 2/2 (100%), human lung giant cell carcinoma 2/2 (100%), human prostate cancer 2?