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为观察全功能隐形眼镜护理液杀灭微生物效果及其毒性,采用悬液定量杀菌试验、动物试验及模拟现场等方法在实验室进行了观察。结果,以其原液作用 10min,对大肠杆菌、金黄色葡萄球菌、绿脓杆菌的平均杀灭率均大于 99. 90%;对白色念珠菌作用 8h,对茄科镰刀霉菌作用 12h,平均杀灭率均大于 90. 00%。含 25%以上小牛血清对其杀灭大肠杆菌效果有轻度影响,将护理液原液于 37℃存放 90d,对杀灭大肠杆菌的效果无明显变化。该护理液原液小鼠急性经口毒性LD50 >5000mg/kg属于实际无毒类;小鼠骨髓嗜多染红细胞微核试验结果为阴性;对家兔皮肤和眼睛无刺激性;豚鼠皮肤致敏试验结果属弱致敏性。结论,该护理液能快速杀灭细胞繁殖体,对白色念珠菌、茄科镰刀霉菌等真菌具有一定杀灭效果,无刺激性、不致敏。
In order to observe the efficacy and toxicity of the full-function contact lens solution to kill microorganisms, the method of suspension quantitative sterilization test, animal experiment and simulation field were observed in the laboratory. The results showed that the average kill rate of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were all over 99. 90% for 10 min. The effect on Candida albicans was 8h, and the effect on Fusarium solani was 12h, Rate of more than 90.00%. Containing more than 25% of calf serum to kill Escherichia coli has a slight effect, the stock solution of care solution stored at 37 ℃ 90d, the effect of killing E. coli no significant change. The oral liquid of nursing solution acute oral toxicity LD50> 5000mg / kg belongs to the actual non-toxic; mouse bone marrow polychromatic erythrocyte micronucleus test results were negative; no irritation to rabbit skin and eyes; guinea pig skin sensitization test The result is a weak sensitization. In conclusion, the nursing solution can quickly kill the cell propagules and has a certain killing effect on fungi such as Candida albicans, Fusarium solanacearum, etc., and is non-irritating and non-allergenic.