目的探讨细胞因子和抗Pgp单抗mAb对K562/ADM耐药细胞株耐药性的逆转作用。方法利用K562细胞的耐阿霉素细胞株K562/ADM采用免疫细胞化学染色法和流式细胞术分别检测mAbMRK-16及rhGM-CSF、rhM-CSF或TNF-α对其Pgp表达的影响。结果将TNF-α100kU/L、rhGM-CSF10μg/L或rhM-CSF10μg/L分别单独与K562/ADM细胞孵育48hPgp表达细胞的阳性率分别为72.19%、80.04%和70.30%与未经任何作用的K562/ADM细胞Pgp表达的阳性率77.02%相比较无明显差异P>0.05。mAbMRK-1610mg/L以及mAbMRK-16分别与TNF-α或rhGM-CSF共同作用于K562/ADM细胞后Pgp表达的阳性率分别为67.49%、67.14%和70.56%与K562/ADM细胞的表达率77.02%相比较Pgp的表达率虽有降低但差别不明显P>0.05只有rhM-CSF与mAbMRK-16共同作用Pgp表达的阳性率为64.29%,可明显抑制K562/ADM细胞上Pgp的表达P<0.05。结论rhM-CSF加mAbMRK-16联合应用,具有逆转K562/ADM细胞MDR的作用。 Objective To investigate the reversal effect of cytokines and anti-Pgp monoclonal antibody (mAb) on drug resistance of K562/ADM cell line. Methods The effect of mAbMRK-16, rhGM-CSF, rhM-CSF or TNF-α on the expression of Pgp in K562/ADM cell line with K562 cells was detected by immunocytochemistry and flow cytometry. . Results The positive rates of Pgp-expressing cells were TNF-α 100kU/L, rhGM-CSF 10μg/L or rhM-CSF 10μg/L, respectively. The positive rates of Pgp-expressing cells were 72.19% and 80.04, respectively. The positive rate of Pgp expression in K562/ADM cells with % and 70.30% peptone and no effect was 77.02%. There was no significant difference between the two groups (P>0.05). The positive rates of Pgp expression were 67.49%, 67.14% and 70.56% in mAbMRK-16, 10mg/L and mAbMRK-16, respectively, after co-treatment with TNF-α or rhGM-CSF in K562/ADM cells. K562/ADM and K562/ADM The expression rate of the cells was 77.02%. The expression rate of Pgp was lower than that of Pgp, but the difference was not significant (P>0.05). Only the rhM-CSF and mAbMRK-16 were combined and the positive rate of Pgp expression was 64.29%. The expression of Pgp on K562/ADM cells was significantly inhibited (P<0.05). Conclusion The combination of rhM-CSF and mAbMRK-16 can reverse MDR in K562/ADM cells.