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目的 观察脊髓损伤后 ,脊髓神经毡等超微结构的变化和中药髓复康对于这种变化的影响 ;另外 ,希图从脊髓损伤区毛细血管内皮细胞的超微结构方面 ,探查髓复康对大鼠损伤脊髓的保护作用、促修复再生作用及其作用机理。方法 制备 45只下胸髓右侧半横断损伤的Wistar大鼠模型 ,分为髓复康、补阳还五汤、甲基强地松龙和空白对照 4组 ,另设正常对照组。用透射电镜和图像分析技术观察和分析髓复康对脊髓损伤区神经元和毛细血管内皮细胞超微结构的影响 ,探讨其与受损神经组织修复再生的关系。结果 ①在空白对照组大鼠 ,脊髓损伤区神经元胞体、轴突和树突野的超微结构发生明显的变性。及时服用髓复康可以保护脊髓损伤区的神经元 ,并且促进受损神经元胞体、神经纤维和树突野的修复再生 :②空白对照组大鼠脊髓损伤区毛细血管内皮细胞的饮液小泡数量明显减少 ,而服用髓复康的大鼠 ,饮液小泡的数量增加 ;③髓复康组大鼠的脊髓星形胶质细胞和毛细血管周细胞的反应比较轻微。结论 脊髓损伤后及时服用髓复康可以 :有效地降低继发性脊髓损伤 ,减小脊髓损伤波及的范围 ;增强脊髓损伤区毛细血管内皮细胞的转运功能 ,迅速消除损伤区的水肿 ,对伤区的神经元起保护作用 ;抑制神经胶质和周细胞的反应性增生 ,创造有利于神经元
Objective To observe the ultrastructural changes of spinal cord nerve mats and the influence of Chinese herbal medicine Pikangkang on this change after spinal cord injury. In addition, Hitu from the ultrastructure of capillary endothelial cells in the spinal cord injury area explores Protection of Rat Spinal Cord Injury, Promoting Repair and Regeneration, and Its Mechanism of Action. Methods Forty-five Wistar rat models of inferior thoracic spinal cord injury were prepared and divided into three groups: Jiefukang, Buyang Huanwu decoction, methylprednisolone and blank control group. A normal control group was also established. Transmission electron microscopy and image analysis techniques were used to observe and analyze the effect of piroxicam on the ultrastructure of neurons and capillary endothelial cells in the spinal cord injury area, and to explore the relationship between the repair and regeneration of damaged nerve tissue. Results 1 In the blank control group, the ultrastructure of neurons, axons and dendrites in the spinal cord injury area was significantly degenerated. Timely administration of piroxicam can protect neurons in the spinal cord injury area and promote the repair and regeneration of injured neurons, nerve fibers and dendritic fields: 2 The control group of rats in the spinal cord injury capillary vascular endothelial cells The number was significantly reduced, while the rats taking fulvaryukol increased the number of drinking vesicles; 3 the spinal cord astrocytes and capillary pericytes of rats in the Minfukang group had a slight response. Conclusion The timely use of piroxicam after spinal cord injury can effectively reduce the secondary spinal cord injury and reduce the range of spinal cord injury. It can increase the transport function of capillary endothelial cells in the spinal cord injury area and quickly eliminate the edema in the injured area. The neurons play a protective role; inhibit the reactive proliferation of glial and pericytes and create favorable neurons