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目的:建立高效液相色谱法同时测定宽筋藤药材中药根碱、巴马汀和小檗碱3个生物碱成分的含量,并对14个不同地区商品药材进行含量测定。方法:采用Sunfire C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,以乙腈(A)-水(0.05 mol·L-1磷酸二氢钾溶液,加0.1%磷酸)(B)为流动相梯度洗脱(0~15 min,20%A→32%A;15~25 min,32%A),流速1.2 m L·min-1,检测波长340 nm。结果:盐酸药根碱、盐酸巴马汀和盐酸小檗碱3种成分质量浓度分别在0.17~0.86、0.08~0.40和0.05~0.26μg·m L-1范围内呈良好线性关系(r≥0.999 7),平均回收率(n=6)分别为99.3%、101.6%和100.0%。14个不同地区16批商品药材含量测定结果显示3个成分含量差异较大。结论:所建立的方法经方法学验证,可用于宽筋藤药材的质量控制和评价。
OBJECTIVE: To establish a method for the simultaneous determination of three alkaloids in Radix Puerariae by HPLC and determination of three alkaloids in palmatine and berberine. The contents of commercial alkaloids in 14 different regions were determined. Methods: The chromatographic conditions were as follows: using a Sunfire C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile (A) -water (0.05 mol·L-1 potassium dihydrogen phosphate solution plus 0.1% phosphoric acid) The mobile phase was gradient elution (0-15 min, 20% A → 32% A; 15-25 min, 32% A) with a flow rate of 1.2 mL · min-1 and a detection wavelength of 340 nm. Results: The concentrations of jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride were linear within the range of 0.17-0.86,0.08-0.40 and 0.05-0.26μg · m L-1 (r ≥0.999 7), and the average recoveries (n = 6) were 99.3%, 101.6% and 100.0%, respectively. The results of determination of medicinal herbs in 16 batches of 14 different regions showed that the content of 3 ingredients varied greatly. Conclusion: The established method is validated by methodology and can be used for the quality control and evaluation of Radix.