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采用热处理超声干卵抗原环卵沉淀试验结合酶联技术的酶联环卵沉淀试验(ELCOPT),与常规环卵沉淀试验(COPT)、酶联免疫吸附试验(ELISA)和间接血凝试验(IHA)进行了比较。在粪检阳性患者327例中,ELCOPT阳性率为96.0%(314/327),COPT阳性率为94.2%(308/327),P>0.05,无显著意义。平均环沉率前法为27.9%,后法为15.7%,P<0.01,有极显著意义。ELISA阳性率为89.6%(293/327),P<0.01,有极显著意义。随机抽双港大队106例,IHA阳性率为82.1%(87/106),与ELCOPT阳性率92.5%(98/106)比较,P<0.05,有显著意义。用ELCOPT检测43例正常人,结果全部阴性。本文采用板井法ELCOPT,可提前24小时出报告,底物以0.4%联苯胺为宜。本法适宜作为大规模血清流行病学调查的工具。
The results of immunocytochemistry and immunocytochemistry were compared with the results of routine ring-oviposit test (COPT), enzyme-linked immunosorbent assay (ELISA), and indirect hemagglutination test (IHA) using ELCOPT, ) Were compared. Among the 327 patients with positive stool examination, the positive rate of ELCOPT was 96.0% (314/327) and the positive rate of COPT was 94.2% (308/327), P> 0.05, but no significant difference. The mean anterior descending rate was 27.9% before the law, 15.7% after the law, P <0.01, a very significant significance. The positive rate of ELISA was 89.6% (293/327), P <0.01, which was of great significance. A total of 106 cases of double-harbor battalion were randomly selected. The positive rate of IHA was 82.1% (87/106), which was significantly higher than that of ELCOPT (92.5%, 98/106) (P <0.05). Forty-three normal subjects were detected by ELCOPT and the results were negative. In this paper, board well method ELCOPT, 24 hours in advance to report, the substrate to 0.4% benzidine is appropriate. This Law is suitable as a tool for large-scale serological epidemiological investigations.