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目的观察运动训练对缺血性卒中后抑郁(PSD)大鼠的干预作用。方法将50只雄性SD大鼠随机均分为假手术组、卒中组、抑郁组、PSD组和运动组。卒中组采用大脑中动脉栓塞法制备局灶性脑缺血大鼠模型,抑郁组采用孤养结合慢性不可预见性温和刺激制备抑郁大鼠模型,PSD组将以上方法相结合制备PSD大鼠模型,运动组制备PSD大鼠并同时进行4周跑台训练。28d后测定五组大鼠体重、糖水消耗量、旷场实验(OFT)行为学评分,采用高效液相色谱法测定五组大鼠脑内5-羟色胺(5-HT)含量。结果 28d后,PSD组和抑郁组体重、糖水消耗量、OFT评分均低于假手术组(P<0.01或P<0.05);卒中组、抑郁组和运动组大鼠体重、糖水消耗量和OFT评分均较PSD组增加(P<0.01或P<0.05)。与假手术组比较,抑郁组、PSD组大鼠海马和大脑皮质5-HT含量减少(P<0.01);卒中组、抑郁组、运动组大鼠海马和大脑皮质5-HT含量较PSD组增加(P<0.01)。结论运动训练可以改善PSD大鼠的行为,提高大鼠脑内5-HT含量。
Objective To observe the effect of exercise training on ischemic stroke depression (PSD) rats. Methods Fifty male Sprague-Dawley rats were randomly divided into sham operation group, stroke group, depression group, PSD group and exercise group. The cerebral infarction group was given middle cerebral artery occlusion (MCAO) to establish the model of focal cerebral ischemia. The depressive group was treated with solitary and chronic unpredictable mild stimuli to establish the depressive rat model. PSD group was made by the above method, Exercise group PSD rats were prepared and treadmill training was performed for 4 weeks at the same time. After 28 days, the body weight, water consumption and open-field test (OFT) scores of rats in each group were measured. The contents of 5-hydroxytryptamine (5-HT) in rat brain were measured by HPLC. Results After 28 days, body weight, water consumption and OFT scores in PSD group and depression group were lower than those in sham operation group (P <0.01 or P <0.05), body weight, sugar consumption and OFT in stroke group, depression group and exercise group The score was higher than PSD group (P <0.01 or P <0.05). Compared with the sham-operation group, the contents of 5-HT in the hippocampus and cerebral cortex of the depressive group and the PSD group were decreased (P <0.01); the content of 5-HT in hippocampus and cerebral cortex of the stroke group, depression group and exercise group were increased (P <0.01). Conclusion Exercise training can improve the behavior of PSD rats and increase the content of 5-HT in rat brain.