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酶生物传感器检测环境污染应用前景良好。该文采用戊二醛-牛血清白蛋白交联法将锇-聚乙烯基吡啶(Os(bpy)2(PVP)10Cl2,Os-PVP)与辣根过氧化物酶(HRP)依次固定在丝网印刷电极上,制备电流型过氧化氢生物传感器。通过循环伏安法对修饰电极的氧化还原性质进行研究,并采用计时-电流法对传感器的固定化和工作参数进行了研究,以便提高信号响应的灵敏度和稳定性。在最适合条件下,底物在修饰电极表面进行催化反应的表观Michaelis-Menten常数kampp为1.79 mmol/L,并确定以HRP酶为标记物的免疫传感器所采用的底物浓度宜大于20倍的kmapp。
Enzyme biosensors have good prospects for detecting environmental pollution. In this paper, osmium-polyvinylpyridine (Os (bpy) 2 (PVP) 10Cl2, Os-PVP) and horseradish peroxidase (HRP) were immobilized on silk On a screen printed electrode, a current-type hydrogen peroxide biosensor was prepared. The redox properties of the modified electrode were studied by cyclic voltammetry. The immobilization and working parameters of the sensor were studied by using the chronoamperometry in order to improve the sensitivity and stability of the signal response. Under the most suitable conditions, the apparent Michaelis-Menten constant kampp of the substrate for the catalytic reaction on the surface of the modified electrode was 1.79 mmol / L, and the substrate concentration of the HRP enzyme-labeled immunosensor should be greater than 20 times Kmapp