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目的:探讨L-肉碱对老年雄性大鼠阴茎勃起功能和结构的影响。方法:以3个月龄和16个月龄的Wistar雄性大鼠为研究对象,饲喂16个月龄雄性大鼠L-肉碱2个月后,测量大鼠体重及血压。阿朴吗啡皮下注射观察30min内大鼠的勃起次数和打哈欠次数。RT-PCR检测TGF-β1mRNA表达水平。Western blot检测阴茎组织TGF-β1蛋白表达水平。F-actin荧光染色和共聚焦显微镜观察阴茎组织平滑肌与胶原的比例。结果:老年组、老年L-肉碱组大鼠体重和血压比较差异无统计学意义(P>0.05)。老年组勃起次数及打哈欠次数明显少于对照组,差异有统计学意义(P<0.01);老年L-肉碱组中勃起次数及打哈欠次数明显多于老年组,差异有统计学意义(P<0.01)。老年组TGF-β1mRNA相对表达水平明显多于对照组,差异有统计学意义(P<0.01);老年L-肉碱组中TGF-β1mRNA相对表达水平明显少于老年组,差异有统计学意义(P<0.01)。TGF-β1蛋白表达率在老年组中明显多于对照组,差异有统计学意义(P<0.01);老年L-肉碱组中TGF-β1蛋白表达率明显少于老年组,差异有统计学意义(P<0.01)。老年组阴茎组织平滑肌与胶原的比例明显低于对照组,差异有统计学意义(P<0.01);老年L-肉碱组阴茎组织平滑肌与胶原的比例明显多于老年组,差异有统计学意义(P<0.01)。结论:L-肉碱可以通过抑制TGF-β1表达改善大鼠阴茎勃起功能。
Objective: To investigate the effect of L-carnitine on the erectile function and structure of aged male rats. Methods: Wistar male rats aged 3 months and 16 months were fed with L-carnitine, a 16-month old male, for 2 months. Body weight and blood pressure were measured. Apomorphine subcutaneous injection of rats within 30 minutes to observe the number of erections and yawning times. The expression of TGF-β1 mRNA was detected by RT-PCR. Western blot was used to detect the expression of TGF-β1 protein in penile tissue. F-actin fluorescence staining and confocal microscopy of penis tissue smooth muscle and collagen ratio. Results: There was no significant difference in body weight and blood pressure between the aged group and the aged L-carnitine group (P> 0.05). The number of erections and yawning in the elderly group were significantly less than those in the control group (P <0.01). The number of erections and yawning in the elderly L-carnitine group were significantly more than those in the elderly group (P <0.01) P <0.01). The relative expression level of TGF-β1mRNA in the aged group was significantly higher than that in the control group (P <0.01). The relative expression level of TGF-β1mRNA in the aged L-carnitine group was significantly less than that in the elderly group (P <0.01) P <0.01). TGF-β1 protein expression in the elderly group was significantly more than the control group, the difference was statistically significant (P <0.01); the elderly L-carnitine group TGF-β1 protein expression was significantly less than the elderly group, the difference was statistically significant Significance (P <0.01). The ratio of smooth muscle to collagen in penile tissue of aged group was significantly lower than that of control group (P <0.01), and the ratio of smooth muscle to collagen in penile tissue of aged L-carnitine group was significantly more than that of aged group, the difference was statistically significant (P <0.01). Conclusion: L-carnitine can improve penile erectile function in rats by inhibiting the expression of TGF-β1.