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目的:研究芍药苷预处理在模拟缺血再灌注损伤条件下对人脐静脉内皮细胞(HUVECs)细胞间黏附分子(ICAM-1)、血管细胞间黏附分子(VCAM-1)蛋白和p-p38 MAPK表达的影响,探寻其对缺血再灌注损伤保护性作用的发生机制。方法:采集、培养原代HUVECs并分为5组,即正常对照组,模型组,芍药苷低、中、高剂量组(0.078、0.156、0.312mg/m L芍药苷预处理);以ELISA法及Western blot法检测不同浓度芍药苷预处理对ICAM-1、VCAM-1和p-p38 MAPK表达水平的影响。结果:正常培养的HUVECs可以表达ICAM-1、VCAM-1和p-p38MAPK,而模拟缺血再灌注后其表达明显增多,加入芍药苷药液后可有效减少其表达(P<0.01,P<0.05)。结论:芍药苷预处理可通过抑制ICAM-1、VCAM-1、p-p38 MAPK蛋白表达发挥对缺血再灌注损伤组织的保护性作用。
OBJECTIVE: To study the effects of paeoniflorin on the expression of ICAM-1, VCAM-1 and p-p38 in simulated ischemia-reperfusion injury in HUVECs. MAPK expression, to explore its protective effect on the mechanism of ischemia-reperfusion injury. Methods: Primary HUVECs were collected and divided into 5 groups: normal control group, model group, paeoniflorin low, medium and high dose groups (0.078,0.156,0.312mg / m L paeoniflorin pretreatment); ELISA And Western blot were used to detect the effect of different concentrations of paeoniflorin on the expression of ICAM-1, VCAM-1 and p-p38 MAPK. Results: The normal cultured HUVECs could express ICAM-1, VCAM-1 and p-p38MAPK, but the expression of ICAM-1, VCAM-1 and p-p38MAPK were significantly increased after simulated ischemia-reperfusion. 0.05). CONCLUSION: Paeoniflorin preconditioning can exert a protective effect on the tissue of ischemia-reperfusion injury by inhibiting the expression of ICAM-1, VCAM-1 and p-p38 MAPK.