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以超速离心制备的电鳐乙酰胆碱酯酶(AChE)及蛋白A亲和层析纯化的单克隆抗体2E6为材料,以酶抑制法观察了2E6对AChE活性的影响。结果显示,随着2E6量的增大,对AChE的抑制作用逐渐增强。表明2E6是AChE的抑制性单克隆抗体。
The effects of 2E6 on AChE activity were observed by enzymatic inhibition assay using AChE and protein A affinity chromatography purified monoclonal antibody 2E6 prepared by ultracentrifugation. The results showed that with the increase of 2E6, the inhibitory effect on AChE gradually increased. 2E6 is an inhibitory monoclonal antibody to AChE.