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采用随机扩增多态性DNA(RAPD)技术对经过连续4代选育的抗白斑综合症病毒(WSSV)中国明对虾(Fennerope naeuschinensis)(XY4)及未经选育的与XY4来源相同的中国明对虾野生群体(HB)进行分析,以期筛选出与抗病性状相关的分子遗传标记,为进一步的基因定位以及中国对虾的种质资源保护和分子标记辅助育种提供有力的技术支撑和理论依据。共进行220个RAPD单引物和114对双引物的检测,产生标记数目共计2439个。依据标记在群体中出现的频率和变化规律,共筛选出5个可能与抗病相关的特异性标记,对这些特异性标记进行测序并将测序结果进行BLAST分析,发现测得片段的序列与数据库中序列的相似性较低,未能找到与所测序列同源性较高的功能基因。这与利用AFLP技术分析的结果一致。由于选育过程施加人工选择,推论这些特异性标记虽不是抗WSSV分子标记,但可能与抗病性状密切相关。
Random amplified polymorphic DNA (RAPD) technique was used to analyze the resistance of White Spot Syndrome Virus (WSSV) Fennerope naeuschinensis (XY4) and unselected China with the same source of XY4 The analysis of HB in wild shrimp showed that it is possible to screen out molecular genetic markers related to disease resistance and provide strong technical support and theoretical basis for further gene mapping, protection of germplasm resources and molecular marker-assisted breeding of P. shrimp. A total of 220 RAPD single primers and 114 pairs of double primers were detected, resulting in a total of 2439 markers. Based on the frequency and variation of the markers in the population, five specific markers that may be related to resistance were screened out. Sequencing of these specific markers and BLAST analysis of the sequencing results showed that the sequence and the database The sequence similarity is low, failed to find a functional gene with high homology with the tested sequence. This is consistent with the results of AFLP analysis. Due to the artificial selection of breeding process, it is concluded that although these specific markers are not anti-WSSV molecular markers, they may be closely related to disease resistance.