目的:探讨清下解胰方对重症急性胰腺炎大鼠血清促炎细胞因子(TNF-αI、L-6)和抗炎细胞因子(IL-10)的调节作用。方法:将清洁级雄性SD大鼠随机分为对照组、模型A组、治疗A组、模型B组、治疗B组;通过经典胆胰管逆行注射法并改良建立重症急性胰腺炎大鼠模型,肉眼大体观察各组大鼠术后表现及死亡情况,光镜下观察胰腺病理损害的改变,检测血清淀粉酶及TNF-αI、L-6I、L-10水平,并分析各指标的相关性。结果:镜下观察胰腺的病理损害显示,治疗A组、治疗B组分别较模型A组、模型B组显著减轻(P<0.01)。模型A组、治疗A组与对照组相比,淀粉酶及TNF-αI、L-6水平均有显著性差异(P<0.01),且治疗A组较模型A组显著降低(P<0.01);模型B组较模型A组淀粉酶水平显著降低,而TNF-αI、L-6水平显著升高(P<0.01);治疗B组较模型B组淀粉酶及TNF-αI、L-6水平均降低,其中TNF-αI、L-6有显著差异(P<0.01)。模型A组、治疗A组与对照组相比I,L-10有显著性差异(P<0.01),且治疗A组和模型B组较模型A组显著升高(P<0.01),治疗B组较模型B组显著升高(P<0.01)。经相关性分析,淀粉酶水平与病理损害无相关;各组TNF-αI、L-6的变化情况一致,呈正相关,且与病理损害呈正相关;IL-10与病理损害和TNF-α无相关。结论:清下解胰方可能通过降低TNF-α与IL-6水平、升高IL-10水平调节促炎细胞因子和抗炎细胞因子,维持高水平的动态网络平衡,阻断“瀑布样级联反应”而起到减轻胰腺损伤的作用。 Objective: To investigate the regulatory effect of Qingxia Xieyao Recipe on serum pro-inflammatory cytokines (TNF-αI, L-6) and anti-inflammatory cytokines (IL-10) in rats with severe acute pancreatitis. Methods: The male SD rats of clean grade were randomly divided into control group, model group A, treatment group A, model group B, and treatment group B. Through the classic retrograde injection of bile duct and pancreatic duct, the rat model of severe acute pancreatitis was established, The gross appearance and death of the rats in each group were observed with the naked eye. The pathological changes of the pancreas were observed under light microscope. The levels of serum amylase, TNF-αI, L-6I and L-10 were measured. Results: Microscopic observation of pathological lesions of the pancreas showed that the treatment A group and the treatment B group were significantly reduced (P <0.01) compared with model group A and model group B respectively. The levels of amylase, TNF-αI and L-6 in model group A and treatment A group were significantly lower than those in control group (P <0.01), and were significantly lower in treatment A group than in model group A (P <0.01) (P <0.01). Compared with model group A, the amylase level and the level of TNF-αI and L-6 in model group B were significantly lower than those in model group A (P <0.01) .There was a significant difference between TNF-αI and L-6 (P <0.01). There was a significant difference in I and L-10 between model group A and treatment A group and control group (P <0.01), and treatment A group and model group B group were significantly higher than model group A (P <0.01) Group than the model group B was significantly higher (P <0.01). Correlation analysis showed that the level of amylase had no correlation with pathological damage. The changes of TNF-αI and L-6 in each group were consistent and positively correlated with the pathological changes, while there was no correlation between IL-10 and pathological damage and TNF-α . Conclusion: Clear down the decoction may be through the reduction of TNF-α and IL-6 levels, increased IL-10 levels regulate proinflammatory cytokines and anti-inflammatory cytokines, to maintain a high level of dynamic network balance, blocking the “waterfall-like Cascade reaction ”and play a role in reducing pancreatic damage.