作者用过氧化物酶标记的蛋白 A,取天疱疮、类天疱疮病变皮肤作免疫电镜研究。其方法是:取1～2mm~3大小的标本,用磷酸盐缓冲盐水 PBS(0.15M,pH7.2)洗,然后在含有蛋白 A-过氧化物酶(0.1mg/ml)、0.5％BSA 以及葡萄糖、半乳糖、甘露糖(各0.03M )的 PBS 中室温下孵育1小时,再用 PBS 洗3次,每次15分钟,接着用Karnovsky 氏甲醛一戍二醛(1∶1稀液)固定液固定 The authors used peroxidase-labeled protein A, pemphigus, pemphigoid skin lesions for immunoelectron microscopy. The method comprises the following steps: taking a specimen of 1-2 mm in size and washing with phosphate buffered saline PBS (0.15 M, pH 7.2), and then culturing in a solution containing protein A-peroxidase (0.1 mg / ml), 0.5% BSA And 0.03% glucose, galactose and mannose (0.03 M each) for 1 hour at room temperature and washed three times with PBS for 15 minutes each, followed by dialysis with Karnovsky’s formaldehyde (1: 1 dilution) Fixed liquid is fixed