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目的建立一种新型大鼠脊髓半切模型,为临床脊髓空洞损伤研究提供适宜的动物模型。方法 25只SD大鼠随机分为脊髓楔型块状半切组(A组,n=10)、假手术组(B组,n=10)和正常对照组(C组,n=5)。术后采用BBB运动功能评分、斜板试验、HE染色和核磁共振(MRI)检测综合评价脊髓半切模型的稳定性。结果术后A组大鼠伤侧后肢BBB运动功能评分及斜板维持最大角度值显著低于B组和C组(P<0.05),而这些指标在B组和C组之间没有明显差异;术后28 d HE和MRI检测显示脊髓损伤区(A组)灰质和白质发生明显的形态学改变。结论通过该方法建立的脊髓半切模型稳定、可靠,适于脊髓空洞性损伤的研究。
Objective To establish a new rat model of spinal cord hemisection, to provide a suitable animal model for the study of clinical syringomyelia injury. Methods Twenty - five Sprague - Dawley rats were randomly divided into two groups: group A (n = 10), group B (n = 10), group C (n = 5). The stability of the spinal cord hemisection model was evaluated comprehensively by BBB motor function score, oblique plate test, HE staining and magnetic resonance imaging (MRI). Results The scores of BBB motor function and maximum angle of slope maintained in the injured hind limbs in group A were significantly lower than those in groups B and C (P <0.05), but there was no significant difference between group B and group C; At 28 days after operation, HE and MRI showed significant morphological changes of gray matter and white matter in the spinal cord injury area (group A). Conclusion The hemisection of spinal cord model established by this method is stable and reliable and is suitable for the study of cavitary injury of the spinal cord.