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目的:研究形成汞膜修饰玻碳电极的最佳条件及该修饰电极用于药物文拉法新的测定。方法:在含有汞离子的1.0mol·L-1硫酸溶液中,采用在负电位下还原的方法,研究了在玻碳电极(GCE)上修饰汞膜的最佳条件;用循环伏安法测定文拉法新。结果:在玻碳电极上修饰汞膜的最佳条件是:在含有2.0×10-4mol·L-1汞离子的1.0mol·L-1硫酸溶液中,在-0.8V电位还原50s。用循环伏安法测定文拉法新(VEN),在汞膜玻碳电极上VEN的氧化电位为0.33V,且峰电流比在裸玻碳电极要大得多。在测定VEN时,多巴胺、抗坏血酸、葡萄糖、尿素以及一些常见碱金属和碱土金属阳离子及阴离子对文拉法新的测定没有干扰,Ag+、Sb3+、Bi3+等干扰严重。测定VEN的线性范围为:1.5×10-6～6.0×10-4mol·L-1,检出限为8.0×10-7mol·L-1。结论:制得的汞膜玻碳电极具有较好的重现性,且制作方法简便,表面更新容易。用于VEN的测定具有较宽的线性范围和较低的检出限,建立了一种测定VEN的新方法。
Objective: To study the optimum conditions for the formation of mercury film modified glassy carbon electrode and the modified electrode for the determination of venlafaxine. Methods: The optimal conditions for the modification of mercury film on glassy carbon electrode (GCE) were investigated by reducing at negative potential in 1.0 mol·L-1 sulfuric acid containing mercury ion. The cyclic voltammetry Venlafaxin. Results: The best conditions for the modification of the mercury film on the glassy carbon electrode were as follows: the solution was reduced at -0.8V for 50s in 1.0mol·L-1 sulfuric acid solution containing 2.0 × 10-4mol·L-1 mercury ions. The voltammetry of venlafaxine (VEN) was determined by cyclic voltammetry. The oxidation potential of VEN was 0.33V at mercury glassy carbon electrode and the peak current was much larger than that of bare glassy carbon electrode. In the measurement VEN, dopamine, determination of ascorbic acid, glucose, urea, as well as common alkali metal and alkaline earth metal cations and anions of venlafaxine of no interference, Ag +, Sb3 +, Bi3 + and other serious interference. The linear range of VEN was 1.5 × 10-6 ~ 6.0 × 10-4mol·L-1 and the detection limit was 8.0 × 10-7mol·L-1. Conclusion: The prepared mercury glassy carbon electrode has good reproducibility, and the preparation method is simple and the surface is easy to renew. The determination of VEN has a wide linear range and a low detection limit, and a new method for determination of VEN was established.