本文继用旋转函数方法对白鲢鱼胰岛素分子在P2_12_12_1晶胞中的取向分析之后,以已知的二锌猪胰岛素分子为模型,应用平移函数及R因子搜索方法,确定了白鲢鱼胰岛素六聚体在晶胞中的位置。在这样得到的模型晶胞中,分子的堆积合理。利用现有的3.5衍射数据,对该模型进行了初步的约束参数最小二乘修正。用修正过的原子参数算得的电子密度图上,未投入修正的多肽链片段有相应的表现。 Following the rotation function method, the orientation of silver carp insulin molecules in P2_12_12_1 cell was analyzed. Using the known zinc dieptaglin molecule model, the shift function and R factor search method were used to determine the silver carp insulin hexamer Body in the unit cell location. In the unit cell thus obtained, the accumulation of molecules is reasonable. Using the existing 3.5 diffraction data, the model was first modified by the least squares method. On the electron density map calculated from the corrected atomic parameters, the unmodified polypeptide chain fragments have corresponding performance.