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探讨肾康注射液对肾小管上皮细胞LLC -PK1分泌纤维连接蛋白 (FN)的影响。采用酶连免疫吸附测定 (ELISA) ,以单味大黄注射液为实验对照组 ,检测肾康注射液对肾小管上皮细胞LLC PK1分泌FN含量的影响。结果显示 :肾康注射液可以显著抑制肾小管上皮细胞LLC PK1分泌FN的含量 ,并呈剂量依赖关系 ;肾康注射液作用明显优于同等含量的单味大黄注射液。说明肾康注射液抑制肾小管上皮细胞LLC PK1分泌FN含量 ,是该方延缓慢性肾功能衰竭进展的机理之一。
To explore the effect of Shenkang injection on the secretion of fibronectin (FN) in renal tubular epithelial cells LLC-PK1. The effect of Shenkang injection on the secretion of FN in LLC PK1 was examined by enzyme linked immunosorbent assay (ELISA) and Danwei Dahuang injection as the experimental control group. The results showed that Shenkang injection can significantly inhibit the secretion of FN from LLC PK1 in renal tubular epithelial cells in a dose-dependent manner. The effect of Shenkang injection was significantly better than that of the same content of Danwei Dahuang injection. It was shown that Shenkang injection inhibited the secretion of FN by LLC PK1 in renal tubular epithelial cells, which was one of the mechanisms by which this side delayed the progression of chronic renal failure.