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目的:探讨在体外受精-胚胎移植(in-vitro fertilization-embryo transfer,IVF-ET)过程中,卵裂期胚胎冷冻10年后解冻复苏再移植成功妊娠的可能性。方法:1例患者卵裂期胚胎冷冻10年后解冻复苏并行囊胚培养,获得囊胚后再次冷冻1年后复苏,行囊胚移植。结果:该患者移植后顺利获得临床妊娠,并于孕38周剖宫产一健康男婴。结论:长期保存的胚胎解冻后重新冷冻对最终获得妊娠依然有价值;冷冻胚胎囊胚培养有助于提高卵裂期胚胎冷冻复苏后的利用价值及提高胚胎着床率。
OBJECTIVE: To explore the possibility of thawed, resuscitated and transplanted successful cleavage embryos 10 years after in vitro fertilization-embryo transfer (IVF-ET). Methods: One case of cleavage stage embryos was thawed and resuscitated after 10 years of freezing. The blastocysts were cultured in vitro. After blastocysts were obtained, they were frozen again for 1 year and then resumed. Blastocyst transplantation was performed. Results: The patient successfully received clinical pregnancy after cesarean section and 38 healthy cesarean section. CONCLUSION: Cryopreservation of long-term preserved embryos thawed after thawing is still valuable for the final pregnancy. Cryopreservation of frozen embryos helps to improve the utilization value of cryopreservation embryos and increase embryo implantation rate.