Fluorescence analysis applied in the study of lactic acid bacteria (LAB) provides a new method and theory to study probiotics and realize the detection and identification of the strains. It is also possible to achieve automation and computerization. In th
发展适用于低光子数应用环境的荧光寿命分析方法对快速荧光寿命显微成像(FLIM)方法的发展和应用都具有重要意义。受高密度单分子定位显微成像中压缩感知算法的启发,将荧光寿命分析看成一个稀疏逆问题,提出了一种基于交替下降条件梯度(ADCG)的荧光寿命分析新方法,并通过对模拟数据和实验数据进行分析,验证了ADCG-FLIM算法即使在低光子数情形下依然能够较好地分析荧光寿命,从而有利于活细胞快速FLIM技术的发展和应用。
针对化学方法会破坏指纹样本的问题,提出了一种紫外成像的光学方法。基于氨基酸和尿素的相对浓度关系以及散射光谱曲线理论,推导了指纹在不同紫外波段的辐亮度比值与时间关系的数学模型。搭建了一套紫外多光谱成像设备,在3个紫外波段对指纹进行了无损提取。对不同波段指纹图像的DN值进行反演分析,得到了不同波段的DN值之比与指纹遗留时间之间的关系。实验结果表明:各紫外波段的DN值之比仅与时间有关,与指纹个体差异无关。指纹老化曲线拟合的表达式与推导的数学模型一致,验证了紫外多光谱成像对指纹遗留时间评估的可行性,为无损指纹测龄
Laser absorption spectroscopy (LAS) has become the most widely used laser spectroscopic technique for gas detection due to its capability of accurate quantification and straightforward operation. However, since resolving weak absorption and averting over-
A diode-end-pumped tunable twisted-mode cavity Tm, Ho:YAG laser with single-longitudinal-mode (SLM) operation is demonstrated in this Letter. The maximal SLM output power is 106 mW with a slope efficiency of 4.86%. The wavelength can be changed from 2090.
In order to reveal the evolution mechanism of repaired morphology and the material’s migration mechanism on the crack surface in the process of
By using a spherical wave as the reference wave, we recorded the in-line phase-shifting digital hologram of the 25th element of Chinese standard No. 3 resolution test pattern, and gave the corresponding numerical reconstructed results. Some problems conce
本文报导提高室温封离式选支CO激光器工作寿命的实验结果,以及影响寿命的一些因素。连续运转寿命超过1万小时。在本实验条件下能够选谱线104条,强线功率达3.5瓦。
在场流体模型的基础上,建立一个描写光束衍射传输(旁轴)的计算机程序。