核糖核酸酶A(RNase A)水解核糖核酸的机制前人已有研究.Markham等和Brown等根据水解过程中有2′,3′-环核苷酸的生成提出了两步机制,即磷酰基转移和水解开环.Witzel等用紫外差值(ΔA_(286))光谱和pH-stat(pH恒定器)技术进行动力学研究,所得的结果支持了上述机制.Williams用同样的方法进行研究,提出除了两步机制外,还有另一途径,即二核苷(3′→5′)单磷酸二酯(A)不经过2′,3′-环核苷酸(B)而直接转化为3′-核苷酸 The mechanism of ribonuclease A (RNase A) hydrolyzing ribonucleic acid has been studied in the past.Markham et al. And Brown et al. Proposed a two-step mechanism based on the generation of 2 ’, 3’- Transfer and hydrolysis of ring-opening .Witzel, etc. with the UV difference (ΔA_ (286)) spectroscopy and pH-stat (pH constant) technology for kinetic studies, the results obtained support the above mechanism.Williams using the same method to study, In addition to the two-step mechanism, it is proposed that the dinucleoside (3 ’→ 5’) monophosphate diester (A) is converted directly to 3’-nucleotides