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目的监测赣州市禽类养殖人群和环境高致病性禽流感病毒的感染情况,为其预防控制策略的制定提供科学依据。方法根据《全国职业暴露人群血清学和环境高致病性禽流感监测方案》的要求,采用单放射免疫扩散溶血试验(SRH)或马红细胞血凝抑制试验(HI)检测禽类养殖人群H5N1血凝素抗体;采用Real-time PCR对环境标本进行A型/和H5、H7、H9亚型禽流感病毒核酸检测。结果 2009年-2013年5个监测年度共657份禽类养殖人群血清H5N1血凝素抗体均为阴性;621份环境标本禽流感病毒核酸监测A型阳性率为15.78%(其中H5阳性率5.96%,H7阳性率0.97%,H9阳性率0.97%,A未分型8.86%,且H7和H9是混合感染)。结论赣州市禽类养殖监测点环境中存在H5、H7、H9禽流感病毒,存在人感染高致病性禽流感的风险,应加强禽类养殖人群的禽流感病毒感染监测。赣州市尚无人感染H5N1禽流感。
Objective To monitor the infection status of poultry breeding population and environmental HPAI in Ganzhou and provide a scientific basis for its prevention and control strategy. Methods According to the requirements of the National Occupational Exposure Program of Serological and Environmental Pathogenic Avian Influenza Surveillance, single-radioimmunoassay hemolysis (SRH) or equine hemagglutination inhibition (HI) was used to detect the blood coagulation of H5N1 The antibodies against A / H and H5, H7 and H9 subtype avian influenza virus were detected by Real-time PCR in environmental samples. Results Serum H5N1 hemagglutinin antibodies were negative in 657 bird farms during the five monitoring years from 2009 to 2013. The positive rate of type A nucleic acid in 621 environmental samples was 15.78% (the positive rate of H5 was 5.96% H7 positive rate 0.97%, H9 positive rate 0.97%, A non-typing 8.86%, and H7 and H9 are mixed infections). Conclusion H5, H7 and H9 avian influenza viruses are found in the environment of poultry breeding monitoring sites in Ganzhou. There is a risk of human being infected with HPAI, and the monitoring of avian influenza virus infection in poultry breeding populations should be strengthened. No one in Ganzhou City is infected with H5N1 bird flu.