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目的从弯蕊开口箭 (TupistrawattiiHook .f.)中寻找抗癌活性先导化合物。 方法使用稻瘟霉活性筛选体系进行活性追踪分离 ,并通过波谱分析 ,特别是二维核磁手段结合化学方法对分离得到的化合物进行结构鉴定。结果从弯蕊开口箭中分离得到 3个具有很强细胞毒活性的强心苷 ,其中化合物 2wattosideF是一个新化合物 ,并且它的细胞毒活性在 3个强心苷中最强 ,其IC50 值为 0 0 11μmol/L。 结论wattosideF是一个具有很强细胞毒活性的新的强心苷类化合物。
Objective To search for anticancer active lead compounds from TupistrawattiiHook.f. Methods The active screening system was used to isolate and isolate the active compounds. The structures of the isolated compounds were identified by spectroscopic analysis, especially two-dimensional nuclear magnetic method combined with chemical methods. Results Three cardiac glycosides with strong cytotoxic activity were isolated from the arrowhead, and the compound 2wattosideF was a new compound, and its cytotoxic activity was the strongest among the three cardiac glycosides with IC50 values. 0 0 11μmol/L. Conclusion Watthoside F is a novel cardiac glycoside with a strong cytotoxic activity.