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目的:探讨海人酸诱导大鼠颞叶癫(EP)发作后2种γ-氨基丁酸(GABA)受体亚单位GABABR亚单位1a(GBR1a)和GABABR亚单位2(GBR2)在EP发生、发展中的作用。方法:运用原位杂交及免疫组化法,检测EP发作后GABABR亚单位mRNA及蛋白在海马的表达。结果:致早期CA1和CA3区2种亚单位mRNA表达持续低下后逐渐增加,DG区则暂时性下降后很快回升;而免疫反应早期却未见明显改变,随后CA1和CA3区表达处于低水平,DG区和颞叶皮质表达下降后很快恢复。结论:致后2种GABAB受体亚单位基因和蛋白表达上调为颞叶EP的内源性自我保护机制。
Objective: To investigate the effects of GABA receptor subunit 1a (GBR1a) and GABABR subunit 2 (GBR2) on the occurrence of EP after kainate induced rat temporal temporal epilepsy (EP) The role of development. Methods: In situ hybridization and immunohistochemistry were used to detect the expression of GABABR subunit mRNA and protein in hippocampus after EP onset. Results: The mRNA expression of the two subunits in early CA1 and CA3 regions gradually increased, while in DG region, the expression of mRNA in the subregions of CA1 and CA3 rose rapidly. However, the expression of CA1 and CA3 remained low at the early stage of immune response. , DG area and temporal cortex expression decreased quickly restored. CONCLUSION: The up-regulation of the expression of two GABAB receptor subunit genes and proteins is the endogenous self-protection mechanism of temporal lobe EP.