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目的探讨高压氧联合rh-EPO对窒息新生大鼠心肌细胞caspase-3表达及细胞凋亡的影响,为临床治疗新生儿窒息后心肌损害提供分子理论依据。方法将新生3~7 d的健康SPF级Wistar大鼠随机分为对照组、窒息组、rh-EPO组、高压氧组、高压氧联合rh-EPO组,每组8只,分别给予相应的干预措施后,采用免疫组织化学染色法检测Caspase-3蛋白表达水平,采用TUNEL染色检测心肌细胞的凋亡率。结果与rh-EPO组和高压氧组比较,高压氧联合rh-EPO组可明显降低窒息新生大鼠心肌细胞caspase-3的表达和细胞凋亡,差异有统计学意义(P<0.05)。结论新生大鼠窒息后采用高压氧及rh EPO联合治疗可明显减轻窒息新生大鼠心肌细胞的损害。
Objective To investigate the effects of hyperbaric oxygen combined with rh-EPO on caspase-3 expression and apoptosis in neonatal rat asphyxia neonatal rats, and to provide a molecular basis for the clinical treatment of neonatal asphyxia myocardial damage. Methods Healthy SPF Wistar rats of 3 ~ 7 d were randomly divided into control group, asphyxia group, rh-EPO group, hyperbaric oxygen group, hyperbaric oxygen group and rh-EPO group, with 8 rats in each group. After treatment, the expression of Caspase-3 protein was detected by immunohistochemical staining, and the apoptosis rate of cardiomyocytes was detected by TUNEL staining. Results Compared with rh-EPO group and hyperbaric oxygen group, hyperbaric oxygen combined with rh-EPO group could significantly reduce caspase-3 expression and apoptosis in asphyxial neonatal rat cardiomyocytes, the difference was statistically significant (P <0.05). Conclusion Newborn rats asphyxia with hyperbaric oxygen and rh EPO combined treatment can significantly reduce asphyxial neonatal rat cardiomyocyte damage.