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目的 克隆人食管癌发生相关的基因。方法 用ｍ Ｒ Ｎ Ａ 差异显示技术分离、克隆食管癌组织中不表达或低表达的ｃ Ｄ Ｎ Ａ 片段，再通过 Ｎｏｒｔｈｅｒｎ ｂｌｏｔ 、ｄｏｔ ｂｌｏｔ 和 Ｒ Ｔ Ｐ Ｃ Ｒ 证实。结果 获得２８０ｂｐ 的ｃ Ｄ Ｎ Ａ 片段，命名为 Ｃ６２ Ａ。与 Ｇｅｎ Ｂａｎｋ 基因数据库比较，未发现 Ｃ６２ Ａ 与任何已知基因有同源性。查询 Ｅ Ｓ Ｔ 数据库，发现 Ｃ６２ Ａ 与ｎｅ２７ｂ０３ ，ｓ１ Ｎ Ｃ Ｉ Ｃ Ｇ Ａ Ｐ Ｃ０３ 人ｃ Ｄ Ｎ Ａ 克隆８９８５４１ 及人卵巢肿瘤ｃ Ｄ Ｎ Ａ 克隆７５５１９６ 等高度同源。 Ｎｏｒｔｈｅｒｎ ｂｌｏｔ 结果显示６／６ 例食管癌组织表达丧失或低表达，ｄｏｔ ｂｌｏｔ 分析表明７／８例食管癌组织低表达， Ｒ Ｔ Ｐ Ｃ Ｒ 显示食管癌细胞系 Ｅ Ｃ１０９ 、 Ｅ Ｃ８７１２ 、 Ｅ Ｃ９７０６ 和肺腺癌细胞系 Ｇ Ｌ Ｃ８２ 极弱表达，１７／２０ 例食管癌组织表达丧失或低表达，胎儿食管、皮肤、大脑、胎盘较高表达，胎儿胃、肝较弱表达，胎儿心、小肠、肾不表达。结论 在食管癌细胞系和食管癌组织高频率的不表达或低表达提示， Ｃ６２ Ａ 很可能与食管癌的发生、发展有关。
Objective To clone gene related to human esophageal cancer. METHODS: mD N A differential display technique was used to isolate and clone c D N A fragments that were not expressed or were lowly expressed in esophageal cancer tissues. The results were confirmed by Northern blot, dot blot and RT-PCR. Results A 280 bp cD N A fragment was obtained and named C6-2A. Compared with the Gen Bank gene database, no homology was found between C6-2A and any known gene. By querying the E S T database, we found that C6-2 A and ne27b03, s1 N C I C G A P C03 human c D N A clone 898541 and human ovarian tumor c D N A clone 755196 were highly homologous. Northern blot results showed that 6/6 cases of esophageal cancer tissue expression loss or low expression, dot blot analysis showed that 7/8 cases of esophageal cancer tissue low expression, R T P C R showed esophageal cancer cell lines E C109, E C8712, E C9706 And lung adenocarcinoma cell line G L C 82 was extremely weakly expressed, 17/20 cases of esophageal cancer tissue expression was lost or low expression, fetal esophageal, skin, brain, placenta higher expression, fetal stomach, liver weak expression, fetal heart The small intestine and kidney do not express. Conclusions The high frequency of esophageal cancer cell and esophageal cancer tissue expression or low expression suggests that C6 2 A is likely related to the occurrence and development of esophageal cancer.