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目的 观察人Po - 5’ -flanking介导的 2 1.5Ku髓鞘碱性蛋白 (myelinbasicprotein ,MBP)微基因 (暂命名为pSVPoMcat)修饰雪旺细胞 (SC)在鼠脊髓内存活及其基因表达。 方法 12 0只大鼠分为 3组 ,A组为pSVPoMcat微基因修饰SC移植组 ;B组为高纯化SC移植组 ;C组为脊髓损伤 (SCI)对照组。伤后各组分别于 2 ,4,8,12周 (每组每次 10只 ) ,取移植区脊髓切片 ,进行S -10 0蛋白、MBP免疫细胞化学染色及地高辛 (DIG)标记的hMBPE2 cDNA探针的原位杂交 (ISH)测定。 结果 伤后 2 ,4,8,12周 ,A组S - 10 0、MBP染色细胞和ISH细胞差异无统计学意义 (P >0 .0 5 ) ,其中ISH阳性细胞可见髓鞘形成。B组的S - 10 0染色细胞逐步递减 ,差异有非常显著性意义 (P <0 .0 1) ,未发现MBP染色细胞以及ISH细胞。C组未发现任何阳性细胞。 结论 pSVPoMcat微基因修饰SC在鼠损伤脊髓内能长期存活并表达外源基因 ,且有助于受伤脊髓的髓鞘形成。
Objective To investigate the survival and gene expression of human Schwann cell (SC) modified by human Po - 5 ’-flanking mediated Schwann cell (SC) with 2.5 ku myelin basic protein (MBP) microgene (tentatively named as pSVPoMcat). Methods 12 0 rats were divided into 3 groups, group A was pSVPoMcat microgene modified SC transplantation group, group B was high purity SC transplantation group, group C was control group of spinal cord injury (SCI). After injury, the spinal cord sections of the grafted area were harvested at 2, 4, 8, and 12 weeks (10 in each group), and S-10 protein, MBP immunocytochemical staining and digoxin (DIG) In situ hybridization (ISH) assay of hMBPE2 cDNA probe. Results There were no significant differences in the number of S - 100, MBP - stained cells and ISH cells in group A at 2, 4, 8 and 12 weeks after injury (P> 0.05), of which ISH positive cells showed myelination. The number of S - 100 stained cells in group B gradually decreased, with significant difference (P <0.01). No MBP - stained cells and ISH cells were found in group B. C group did not find any positive cells. Conclusion pSVPoMcat microgene modified SC can survive long-term and express foreign genes in injured spinal cord, and contribute to myelination of injured spinal cord.